Application

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

This Anti-PTB Antibody, clone BB7 is validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation for the detection of PTB .

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PTB in 10 µg of HT-29 and HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PTB in HeLa, HUVEC, and NIH/3T3 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated PTB in HeLa and WERI-1 nuclear extracts. (Chou, M.Y., et al. (2000). Mol Cell. 5(6):949-57).
Western Blotting Analysis: A representative lot detected PTB in HeLa and WERI-1 nuclear extracts (Sharma, S., et al. (2005). Mol Cell. 19(4):485-496).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Polypyrimidine tract-binding protein 1 (UniProt P26599; also known as 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, Heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, PTB, RNA-binding protein) is encoded by the PTBP1 (also known as HNRNP-I, HNRNPI, HNRPI, PTB, PTB2, PTB3, PTB4, PTB-1, PTB-T) gene (Gene ID 5725) in human. The serine–arginine rich (SR) proteins and the heterogeneous nuclear ribonucleoproteins (hnRNPs) are the most typical families of RBPs involved in splicing regulation. SR proteins generally play a positive role in exon recognition by binding exonic and intronic enhancers near the alternative splice sites. On the other hand, hnRNPs often bind to silencer elements and interfere with the recruitment of spliceosome components or positive regulators. PTB, also known as hnRNP I, belongs to an hnRNP family of RNA-binding proteins and is involved in several aspects of cellular mRNA metabolism including splicing, RNA stability, and internal ribosome entry site (IRES)-mediated translation of viral and cellular mRNAs. PTB functions as a splicing repressor by preventing recruitment of U2AF65 or correct base pairing of U2 snRNP, or by affecting the interaction of U1 snRNP with other components of the spliceosome.

Immunogen

Recombinant protein human PTB.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PTB in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~57 kDa observed. The target band may appear as a doublet, most likely due to the presence of spliced isoforms. Isoform 1 (57.2 kDa calculated), isoform 2/PTB2 (59.0 kDa calculated), isoforms 3 (59.6 kDa calculated).